Mouse Tissue Lysates - Brain Major Regions

Tissue Lysates are Ideal for biomarker and antibody screening/ characterization, protein-protein interaction, protein expression, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting.  We provide the lysates from the following regions of the brain tissues:

Total Brain • Brain-stem • Cerebellum • Cerebral Cortex (Frontal) • Cerebral Cortex (Posterior) • Whole Cerebral Cortex • Hippocampus • Hypothalamus • Medulla Oblongata • Thalamus

Mouse tissues were collected and flash frozen within 5-10 minutes of removal. Tissue total protein lysates are prepared from whole tissue homogenates by homogenization in modified RIP buffer along with protease and phosphatase inhibitor cocktail, and provided at a concentration of 2-5 mg/ml.

The quality of protein is tested by SDS-PAGE with coomassie blue staining of the gel. Proteins from one of gel was transferred to PVDF membrane and tested with beta actin antibody.

Components

• Tissue Lysates (2-5 mg ⁄ ml)
• Instruction Manual

Storage and Stability

From time of receipt, this product is stable for SIX months at – 20°C or 12 months at – 70°C.

DISCLAIMER

FOR IN - VITRO RESEARCH USE ONLY - NOT RECOMMENDED FOR DIAGNOSTIC OR THERAPEUTIC USAGE